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This is a nice experiment which rewards careful preparation. It helps if the spots are small but concentrated. This means ideally drying each starting spot and re-spotting in the same place two or three times. The solvent tends to rise quite slowly, so unless you have a whole morning or afternoon session you will have to allow the students to leave their chromatograms rising and remember to remove them and mark on the solvent front.
Materials:
| 0.01% glycine (1 drop) | 0.01% alanine (1 drop) |
| 0.01% arginine (1 drop) | 0.01% aspartic acid (1 drop) |
| 0.01% phenylalanine (1 drop) | 0.01% tyrosine (1 drop) |
| Butan-1-ol (12 cm3) | Ethanoic acid, glacial (3 cm3) |
| Ninhydrin spray | Propan-1-ol (1 cm3) |
| Melting point tubing | Bunsen burner |
| Heat-proof mat | Chromatography paper (about 25 x 12 cm) |
| Paper clip | 1000 cm3 beaker |
| 10 cm3 measuring cylinder | 25 cm3 measuring cylinder |
| Cling film | Oven |
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